Neomycin resistance as a selectable marker in Methanococcus maripaludis
نویسندگان
چکیده
منابع مشابه
Neomycin resistance as a dominant selectable marker for selection and isolation of vaccinia virus recombinants.
The antibiotic G418 was shown to be an effective inhibitor of vaccinia virus replication when an appropriate concentration of it was added to cell monolayers 48 h before infection. Genetic engineering techniques were used in concert with DNA transfection protocols to construct vaccinia virus recombinants containing the neomycin resistance gene (neo) from transposon Tn5. These recombinants conta...
متن کاملHydrogenases of Methanococcus maripaludis
The methanogens catalyze a major component of the Earth’s H2 cycle. They are especially active in anaerobic environments where they are the primary consumers of fermentatively produced H2. These strictly anaerobic Archaea have evolved unique adaptations to H2 metabolism, many of which are poorly understood. Our research examines the enzymes and pathways in H2 metabolism in methanogens, especial...
متن کاملTaxis Toward Hydrogen Gas by Methanococcus maripaludis
Knowledge of taxis (directed swimming) in the Archaea is currently expanding through identification of novel receptors, effectors, and proteins involved in signal transduction to the flagellar motor. Although the ability for biological cells to sense and swim toward hydrogen gas has been hypothesized for many years, this capacity has yet to be observed and demonstrated. Here we show that the av...
متن کاملGenetics of nitrogen regulation in Methanococcus maripaludis.
We have used genetic methods in Methanococcus maripaludis to study nitrogen metabolism and its regulation. We present evidence for a "nitrogen regulon" in Methanococcus and Methanobacterium species containing genes of nitrogen metabolism that are regulated coordinately at the transcriptional level via a common repressor binding site sequence, or operator. The implied mechanism for regulation re...
متن کاملCORRIGENDUM: Taxis Toward Hydrogen Gas by Methanococcus maripaludis
The Supplementary Movies that accompany this study were omitted from the original version of this Article.
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1996
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.62.11.4233-4237.1996